Advances in Protein Chemistry

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Front Cover 2
Advances in Protein Chemistry, Volume VIII 5
Copyright Page 6
Contributors to Volume VIII 7
Contents 9
Chapter 1. Naturally Occurring Peptides 13
- Introduction 16
- I. Peptides with Widespread Distribution in Living Cells 22
- II. Peptides of Bacterial Origin 61
- III. Peptides of Plant Origin 87
- IV. Peptides of Animal Origin 100
- V. Conclusions 118
Chapter 2. Peptide Bond Formation 139
- II. Thermodynamic Aspects of Peptide Synthesis 140
- I. Introduction 140

Front Cover 2
Advances in Protein Chemistry, Volume VIII 5
Copyright Page 6
Contributors to Volume VIII 7
Contents 9
Chapter 1. Naturally Occurring Peptides 13
- Introduction 16
- I. Peptides with Widespread Distribution in Living Cells 22
- II. Peptides of Bacterial Origin 61
- III. Peptides of Plant Origin 87
- IV. Peptides of Animal Origin 100
- V. Conclusions 118
Chapter 2. Peptide Bond Formation 139
- II. Thermodynamic Aspects of Peptide Synthesis 140
- I. Introduction 140
- III. Transpeptidation 149
- IV. Amide Transferases 162
- V. Peptide Syntheses Where –ΔF Is Negative and Large: Coupled with High Energy Phosphate 164
- VI. Amino Acid Incorporation into Proteins and Protein Synthesis 168
Chapter 3. Bacteriophages : Nature and Reproduction 187
- I. Introduction 189
- II. Growth and Purification of Bacteriophages 196
- The Virus Particle—Extracellular Phase 196
- III. Criteria of Purity for Bacteriophages 202
- IV. Physical Properties 211
- V. Chemical Composition of E. coli Bacteriophages 229
- VI. Inactivation and Disruption of Coliphages 239
- VII. Summary 247
- The Virus-Host Cell Interaction—Intracellular Reproduction 249
- VIII. Adsorption. 249
- IX. Methods for Study of Bacteriophage Multiplication 252
- X. Isotopic Studies of Coliphage Reproduction 269
- XI. Fate of the Infecting Particle 280
- XII. Summary 285
Chapter 4. Assimilation of Amino Acids by Gram-Positive Bacteria and Some Actions of Antibiotics Thereon 297
- I. Introduction 299
- II. Occurrence of Free Amino Acids in Bacteria and Other Tissues 301
- III. Nature of "Free" Glutamic Acid 304
- IV. Release of Internal Constituents from Bacterial Cells 305
- V. Passage of Metabolites through Semipermeable Membranes 310
- VI. Internal Accumulation of Lysine 311
- VII. Accumulation of "Free" Glutamic Acid within Gram-Positive Bacteria 319
- VIII. Utilization of Derivatives of Glutamic Acid 345
- IX. Accumulation of Other Amino Acids 349
- X. Extracellular Accumulation of Glutamic Acid Peptides 351
- XI. Protein Synthesis in Staphylococcus aurus 356
- XII. Incorporation of Glutamic Acid into Cell Protein without Net Synthesis 362
- XIII. Nucleic Acid Synthesis 364
- XIV. Relation between Nucleic Acid Content and Rates of Protein Synthesis or Accumulation of "Free" Glutamic Acid 366
- XV. Experimental Separation of Stages in Amino Acid Assimilation 368
- XVI. Actions of Antibiotics on Stages of Assimilation of Amino Acids in Staphylococcus aureus 370
- XVII. Speculations 383
Chapter 5. Peanut Protein Isolation, Composition, and Properties 405
- I. Introduction 405
- II. Production and Processing 406
- III. Composition of Peanuts 409
- IV. Solubility and Isolation of the Proteins 409
- V. Composition of Peanut Protein 411
- VI. Physical Chemical Properties of the Proteins 411
- VII. Chemical Reactions of the Proteins 416
- VIII. Peanut Proteins in Foods and Feeds 416
- IX. Nonfood Uses for Peanut Proteins 418
- X. Summary 420
Chapter 6. Rotational Brownian Motion and Polarization of the Fluorescence of Solutions 427
- Introduction 428
- I. Rotational Brownian Motion 431
- II. Fluorescence Polarization 440
- III. Fluorescence of Proteins and Protein Conjugates 453
- IV. Polarization Studies of Conjugates 457
Chapter 7. Zone Electrophoresis 473
- I. Introduction 473
- II. Types of Apparatus 475
- III. Working Conditions 481
- IV. Sources of Error 482
- V. Methods of Zone Localization 484
- VI. Mobilities and Isoelectric Points 487
- VII. Fields of Application 489
- VIII. Preparative Zone Electrophoresis 494
- IX. Electrochromatography 495
- X. Zone Electroultrafiltration 496
Author Index 499
Subject Index 524

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